One-tube, two-stage PCR-directed in vitro mutagenesis using megaprimers
نویسندگان
چکیده
منابع مشابه
A rapid and efficient one-tube PCR-based mutagenesis technique using Pfu DNA polymerase
A rapid method for efficiently generating site-directed mutations on a clean sequence background is described. This modification of the megaprimer PCR mutagenesis approach can be performed in one tube in less than 4.5 hours, and does not require purification of intermediate products. High fidelity of DNA sequence replication is obtained by employing Pfu DNA polymerase and limiting the total num...
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ژورنال
عنوان ژورنال: Technical Tips Online
سال: 1997
ISSN: 1366-2120
DOI: 10.1016/s1366-2120(08)70058-8